Turhaa hösläystä: ei ole luettu perusartikkelia

Risto Koivula, 6/26/2021 9:16:04 AM, 405642

Lainaus: Risto Koivula, 13.06.2021 06:43:06, 405524
Lainaus: Teppolainen, 12.06.2021 23:16:42, 405522


https://www.sciencedirect.com/science/article/pii/S1873506120304165

Stem Cell Research

Volume 50, January 2021, 102115
Stem Cell Research

Furin cleavage sites naturally occur in coronaviruses

Author links open overlay panel
Yiran Wu, a
Suwen Zhao, ab

a Human Institute, ShanghaiTech University, Shanghai 201210, China

b School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China

Received 30 September 2020, Revised 2 December 2020, Accepted 4 December 2020, Available online 9 December 2020.

Abstract

The spike protein is a focused target of COVID-19, a pandemic caused by SARS-CoV-2. A 12-nt insertion at S1/S2 in the spike coding sequence yields a furin cleavage site, which raised controversy views on origin of the virus. Here we analyzed the phylogenetic relationships of coronavirus spike proteins and mapped furin recognition motif on the tree. Furin cleavage sites occurred independently for multiple times in the evolution of the coronavirus family, supporting the natural occurring hypothesis of SARS-CoV-2.



...

2.3. Furin cleavage sites are common in Betacoronavirus

Our mapping results showed that the furin recognition motif is more common in Merbecovirus and Embecovirus (Figs. 4 and S2, S3).

In Merbecovirus, furin sites at spike S1/S2 occur in three clades: MERS-CoV strains, the bat coronavirus HKU5 strains, and coronavirus Neoromicia/PML-PHE1/RSA/2011 with its relatives (Figs. 4A and S2). Besides, MERS-CoV and bat coronavirus HKU5 are the only clades in Merbecovirus having furin cleavage site at S2′. In Embecovirus, furin recognition motif at spike S1/S2 is universal: All strains but a few exceptions have furin cleavage sites at spike S1/S2 (Figs. 4B and S3). Interestingly, the Longquan Aa mouse coronavirus (Wang et al., 2015) loses this furin site, while its close relatives (e.g. China Rattus coronavirus HKU24, sequence identity 96.0%) maintains the furin cleavage site. This provides an example of naturally occurred sequence variation at spike S1/S2 among closely related coronaviruses. Besides, for spike S2′, only several single strains have furin recognition motif (Fig. S3).

2.4.Furin cleavage sites also occur in other genera of coronavirus




Fig. 6. Positions of furin cleavage sites at the linking region of S1 and S2. A) Multiple sequence alignment of representative Betacoronavirus spike protein S1/S2 region, with furin recognition motifs highlighted (red colorboxes in sequence alignment). Phylogenetic tree of spike protein sequences is colored to indicate subgenera (coloring scheme the same as in Fig. 2B). B) Positions of furin cleavage sites in different coronavirus genera (red cartoon, furin recognition motif; red arrow, cleavage site); structures: SARS-CoV-2, PDB ID 6VYB (Walls et al., 2020), with missing loop added; feline coronavirus (FCoV) UU16, homology model based on PDB ID 5SZS (Walls et al., 2016); infectious bronchitis coronavirus (IBV), PDB ID 6CV0 (Shang et al., 2018). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Täysin luokaton aivopieru virusten genetiikan (kökkö?)nobelistilta, v. 1975, David Baltimorelta muka ”furin cleavage site ryhmien keinotekoisuudesta” koronaviruksilla...

https://fi.wikipedia.org/wiki/David_Baltimore

https://www.caltech.edu/about/news/the-debate-over-origins-of-sars-cov-2



There has been renewed discussion and interest into understanding the biological origins of SARS-CoV-2, the virus that has caused the COVID-19 pandemic. Similar viruses before it have been shown to have jumped from animals to humans; yet this link has not yet been definitively found for SARS-CoV-2.

Caltech's David Baltimore, president emeritus and Distinguished Professor of Biology, is a virologist who received the Nobel Prize for his research into viral genetics. Baltimore was an organizer of the first Asilomar Conference on Recombinant DNA held in 1975 to discuss ethics and regulation of biotechnology. We sat down with him to discuss the debate over the origins of SARS-CoV-2.

What are the arguments that suggest SARS-CoV-2 is a naturally evolved virus? What is the evidence that suggests that it may have originated in and accidentally released from a laboratory in Wuhan, China?

The argument that it's a naturally evolved virus is from the belief that through the time of evolution, any sequence of RNA or DNA could evolve.

Biologists have seen what evolution can create: the whole natural world around us. We believe that evolution can do anything. But the fact that evolution might have been able to generate SARS-CoV-2 doesn't mean that that's how it came about. I think we very much need to find out what was happening in the Wuhan Institute of Virology. I think that we can't say for sure yet whether the SARS-CoV-2 virus came from natural origins or if it was genetically manipulated somehow.

Recently you were quoted as saying: "When I first saw the furin cleavage site in the viral sequence, with its arginine codons, I said to my wife it was the smoking gun for the origin of the virus. These features make a powerful challenge to the idea of a natural origin for SARS2." Can you unpack this quote for us?

Let me be clear, even though I used the phrase "smoking gun," I don't really think there's a smoking gun in the genome itself.

Now, within the SARS-CoV-2 genome there is an insertion of 12 nucleotides that is entirely foreign to the beta-coronavirus class of virus that SARS-CoV-2 is in. There are many other viruses in this class, including the closest relative of SARS-CoV-2 by sequence, and none of them have this sequence. The sequence is called the furin cleavage site.

To back up a little bit: In order to infect a cell, the spike protein on the surface of viruses like SARS-CoV-2 needs to first be cut, or cleaved. The cut needn't be terribly exact, but it needs to be cut. Different viruses attract different kinds of cellular "scissors," so to speak, to make this cut; the furin cleavage site attracts the furin protein providing the most efficient way to make a cut. You don't need a furin cleavage site to cut the protein, but it makes the virus more efficiently infectious.

So where did it come from in SARS-CoV-2? There are other viruses that have furin cleavage sites, other coronaviruses, though not the family of beta-coronaviruses. ”

RK: Tämä ei siis ole totta, kuten näkyy.

” So this sequence's nucleotides could have hopped from some other virus. No one has identified a virus that has exactly this sequence, but it could have come from something close, then evolved into the sequence that we see today.

I'm perfectly willing to believe that happened, but I don't think it's the only way that that sequence could have appeared. The other way is that somebody could have put it in there. You can't distinguish between the two origins from just looking at the sequence. So, naturally, you want to know were there people in the virology laboratory in Wuhan who were manipulating viral genetic sequences? It's really a question of history: What happened?

When I first saw the sequence of the furin cleavage site — as I've said, other beta coronaviruses don't have that site — it seemed to me a reasonable hypothesis that somebody had put it in there. Now, I don't know if that's true or not, but I do know that it's a hypothesis that must be taken seriously.

Why is it important to know where the virus originated?

Well, I think we want to know the pathway of generating highly infectious new viruses that could cause pandemics because we want to protect ourselves against this happening again. If it happened by natural means, it means that we have to increase our surveillance of the natural environment. We have to try to find the hosts that provide an ability for the virus to change its sequence, to become more infectious. This would mean we need to keep surveillance on markets, on zoos, on places where viruses could jump from one species to another.

But if SARS-CoV-2 came about by an artificial means, it means we've got to put better defenses around laboratories. I'm not suggesting that it was deliberately released if it came from a laboratory, but we have to realize that whatever a laboratory does might get out of the laboratory and create havoc. It means that work of this sort should only go on in what are called biosafety level 4 laboratories.

In the past, you have spoken a lot about the ethics surrounding gene editing technologies like CRISPR/Cas-9. Though the origins of SARS-CoV-2 are still unclear, are there renewed ethical concerns about manipulating viral genomes?

We have a whole toolbox of ways of manipulating the sequences of viral genomes. Those become much more dangerous if they can get out of the lab and cause trouble. That was the genesis of the first Asilomar meeting in 1975. In that meeting, we were discussing ethics around recombinant DNA technology. We were worried that genetically manipulated things could get out of the laboratory and be dangerous. I must say that, at that time, I didn't take the issue of whether, in the process of laboratory experimentation, we might generate dangerous new organisms as seriously as it now appears to me.

But in order to try to understand crossover events and prepare for the next pandemic, scientists need to be able to study viruses in the lab. How do we balance safety with the potential good that can come from studying viruses?

We want to know what tricks viruses have evolved, because those are useful to us in a lot of ways: They tell us what to keep an eye on, what to watch out for. Viruses are very inventive in that sense. They have all sorts of tricks, many of which we haven't seen in organisms other than viruses. We want to know about all of these so that we can be prepared to counter them.

Work in virology is very important from that point of view and also actually gives us tricks that we can use in designing, for instance, gene therapy vectors that are carriers of beneficial genes or therapeutic molecules. At Caltech, my colleagues are developing these types of viral vector technologies that could treat, for example, Huntington's disease.

When I looked at the world of viruses 20, 30 years ago, I was a younger virologist. It seemed to me that there was very little that viruses did that was good. Most of what they did was bad, caused disease of various sorts, even cancer. Today, there is the ability to manipulate viruses. Researchers can remove the genetic material that makes a virus dangerous, that makes people ill, and instead use the virus as a package to get a desired therapeutic into cells. That's an incredibly powerful, positive thing that viruses can do. They don't naturally treat diseases, but we can manipulate them so that they become vectors that allow us to fight diseases.

For more Caltech experts on COVID-19, visit the Caltech Science Exchange. ”

Muokannut: Risto Koivula, 6/26/2021 9:19:57 AM


"Labrasta karannut virus"   [ 405323 , Otto Mäkelä , 18.05.2021 19:55:11 ]
    "Labrasta karannut virus"   [ 405324 , sherlocker , 18.05.2021 21:04:33 ]
        "Labrasta karannut virus"   [ 405361 , Otto Mäkelä , 24.05.2021 19:05:10 ]
            "Labrasta karannut virus"   [ 405362 , Teppolainen , 25.05.2021 00:32:50 ]
                "Labrasta karannut virus"   [ 405363 , Risto Koivula , 25.05.2021 06:02:32 ]
                    "Labrasta karannut virus"   [ 405367 , Otto Mäkelä , 26.05.2021 11:12:57 ]
                    "Labrasta karannut virus"   [ 405380 , Teppolainen , 28.05.2021 02:01:54 ]
                    ERKON HÖRHÖLÄ BIDENIN SUUHUN: "KORONAN EI OLE HAVAITTU VOIVAN TARTTUA ELÄIMILTÄ IHMISILLE"...   [ 405382 , Risto Koivula , 28.05.2021 07:28:01 ]
                        ERKON HÖRHÖLÄ BIDENIN SUUHUN: "KORONAN EI OLE HAVAITTU VOIVAN TARTTUA ELÄIMILTÄ IHMISILLE"...   [ 405384 , PeP , 28.05.2021 09:55:24 ]
                            ERKON HÖRHÖLÄ BIDENIN SUUHUN: "KORONAN EI OLE HAVAITTU VOIVAN TARTTUA ELÄIMILTÄ IHMISILLE"...   [ 405385 , Risto Koivula , 28.05.2021 11:07:45 ]
                                Ainakin koronaviruksen lähimmät sukulaiset lepakoilla tarttuvat ihmisiinkin.   [ 405386 , Risto Koivula , 28.05.2021 11:52:23 ]
    Myös Yleisradion tiedetoimittaja ottaa vakavasti viruksen laboratorioalkuperän   [ 405402 , Teppolainen , 30.05.2021 13:29:41 ]
        Bidenin USA ja Kiina vastuussa WHO:n korruptiosta   [ 405403 , Teppolainen , 30.05.2021 13:52:47 ]
            WHO on YK:n Yleiskokoksen alainen järjestö: sen ei tarvitse totella muita kuin sitä ja todistettua tiedettä.   [ 405520 , Risto Koivula , 12.06.2021 02:29:34 ]
            Bidenin USA ja Kiina vastuussa WHO:n korruptiosta   [ 405528 , _Wesa_ , 13.06.2021 09:31:29 ]
                Bidenin USA ja Kiina vastuussa WHO:n korruptiosta   [ 405529 , Teppolainen , 13.06.2021 11:38:54 ]
                    Bidenin USA ja Kiina vastuussa WHO:n korruptiosta   [ 405530 , _Wesa_ , 13.06.2021 13:04:09 ]
        Viruksen laboratorioalkuperää pidetään tiedepiireissä epätodennäköisenä   [ 406924 , Otto Mäkelä , 15.09.2021 17:16:50 ]
    USA:n senaatin tiedotustilaisuus kertoo Big Techin sensuurista ja koronaviruksen alkuperästä   [ 405522 , Teppolainen , 12.06.2021 23:16:42 ]
        Turhaa hösläystä: ei ole luettu perusartikkelia   [ 405524 , Risto Koivula , 13.06.2021 06:43:06 ]
            Turhaa hösläystä: ei ole luettu perusartikkelia   [ 405526 , Risto Koivula , 13.06.2021 07:51:11 ]
            Turhaa hösläystä: ei ole luettu perusartikkelia   [ 405642 , Risto Koivula , 26.06.2021 09:16:04 ]
        USA:n senaatin tiedotustilaisuus kertoo Big Techin sensuurista ja koronaviruksen alkuperästä   [ 405531 , _Wesa_ , 13.06.2021 13:28:35 ]
    Tautiset ulkomaalaiset ovat taas ykkössijalla   [ 405577 , Teppolainen , 21.06.2021 08:11:05 ]
        Tautia tuodaan taas bussilasteittain ulkomailta   [ 405605 , Teppolainen , 24.06.2021 11:00:22 ]
            Tautia tuodaan taas bussilasteittain ulkomailta   [ 405609 , Trepidaatio , 24.06.2021 11:54:11 ]
            Tautia tuodaan taas bussilasteittain ulkomailta   [ 405612 , _Wesa_ , 24.06.2021 17:45:18 ]
                Tautia tuodaan taas bussilasteittain ulkomailta   [ 405613 , Teppolainen , 24.06.2021 18:18:39 ]
                    Tautia tuodaan taas bussilasteittain ulkomailta   [ 405614 , _Wesa_ , 24.06.2021 18:30:57 ]
                        Tautia tuodaan taas bussilasteittain ulkomailta   [ 405615 , Teppolainen , 24.06.2021 19:05:08 ]
                            Tautia tuodaan taas bussilasteittain ulkomailta   [ 405616 , _Wesa_ , 24.06.2021 19:15:05 ]
                                Tautia tuodaan taas bussilasteittain ulkomailta   [ 405618 , Teppolainen , 24.06.2021 19:24:37 ]
                                    Tautia tuodaan taas bussilasteittain ulkomailta   [ 405619 , _Wesa_ , 24.06.2021 20:08:27 ]
                                        Tautia tuodaan taas bussilasteittain ulkomailta   [ 405620 , Teppolainen , 24.06.2021 20:32:22 ]
                                            Tautia tuodaan taas bussilasteittain ulkomailta   [ 405625 , _Wesa_ , 25.06.2021 11:44:23 ]
                                                Tautia tuodaan taas bussilasteittain ulkomailta   [ 405627 , Teppolainen , 25.06.2021 12:59:18 ]
                                                    Tautia tuodaan taas bussilasteittain ulkomailta   [ 405631 , _Wesa_ , 25.06.2021 16:05:44 ]
                                                        Tautia tuodaan taas bussilasteittain ulkomailta   [ 405635 , Teppolainen , 25.06.2021 17:38:16 ]
                                                            Suomen hallituksen juoksupojat levittävät koronaa hiki hatussa   [ 405645 , Teppolainen , 26.06.2021 18:58:20 ]
                                                                Suomen hallituksen juoksupojat levittävät koronaa hiki hatussa   [ 405662 , _Wesa_ , 27.06.2021 10:41:20 ]
                                                                    Suomen hallituksen juoksupojat levittävät koronaa hiki hatussa   [ 405667 , Teppolainen , 27.06.2021 13:05:47 ]
                                                                        Suomen hallituksen juoksupojat levittävät koronaa hiki hatussa   [ 405677 , _Wesa_ , 27.06.2021 14:19:52 ]
                                                                            Jalkapallohuligaanit levittävät koronaa hiki hatussa   [ 405688 , Teppolainen , 27.06.2021 15:34:30 ]
                                                                                Jalkapallohuligaanit levittävät koronaa hiki hatussa   [ 405711 , _Wesa_ , 29.06.2021 14:02:11 ]
                                                                                    Jalkapallohuligaanit levittävät koronaa hiki hatussa   [ 405714 , Teppolainen , 29.06.2021 14:22:04 ]
                                                                                        "Mallikasta"   [ 405718 , Trepidaatio , 29.06.2021 14:45:28 ]
                                                                                            "Mallikasta"   [ 405720 , Teppolainen , 29.06.2021 14:57:20 ]
                                                                                                Mallikasta   [ 405722 , Trepidaatio , 29.06.2021 15:00:15 ]
                                                                                            Venäjän koronaonnistumisten vähättely   [ 406028 , Teppolainen , 22.07.2021 11:42:24 ]
                                                                                        Jalkapallohuligaanit levittävät koronaa hiki hatussa   [ 405727 , _Wesa_ , 29.06.2021 20:24:41 ]
                                                            Tautia tuodaan taas bussilasteittain ulkomailta   [ 405661 , _Wesa_ , 27.06.2021 10:32:51 ]
                                                                Tautia tuodaan taas bussilasteittain ulkomailta   [ 405669 , Teppolainen , 27.06.2021 13:13:23 ]
                                                                    Tautia tuodaan taas bussilasteittain ulkomailta   [ 405675 , _Wesa_ , 27.06.2021 14:09:47 ]
                                                                        Tautia tuodaan taas bussilasteittain ulkomailta   [ 405690 , Teppolainen , 27.06.2021 15:38:41 ]
                                                                            Edes tartuttajien henkilöllisyyksiä ei rajalla tarkistettu ja myös THL panttaa tietoja - Enqvist varoittaa fasismista   [ 405703 , Teppolainen , 28.06.2021 11:11:40 ]
                                                                                Pakollinen koronatesti rajalla "unohtuu" viranomaisilta yhä useammin   [ 405709 , Teppolainen , 29.06.2021 11:56:13 ]
                                                                                    Ei määräystä korkeammalta taholta   [ 405717 , Trepidaatio , 29.06.2021 14:43:24 ]
                                                                                        Määräys korkeammalta taholta   [ 405719 , Teppolainen , 29.06.2021 14:50:40 ]
                                                                                            Ei määräystä korkeammalta taholta   [ 405721 , Trepidaatio , 29.06.2021 14:59:12 ]
                                                                                                Määräys korkeammalta taholta   [ 405723 , Teppolainen , 29.06.2021 17:07:02 ]
                                                                                                    ei määräystä korkeammalta taholta   [ 405724 , Trepidaatio , 29.06.2021 17:25:50 ]
                                                                                                        Poliisin totalitaristinen toiminta   [ 405725 , Teppolainen , 29.06.2021 18:43:33 ]
                                                                                                            Poliisi ei saanut ohjeita ylemmältä taholta näitä kahta testauksestakiekieltäytyjää varten   [ 405726 , Trepidaatio , 29.06.2021 18:56:21 ]
                                                                                                                Poliisin ohjeet ylemmältä taholta myös näitä kahta koskien   [ 405734 , Teppolainen , 30.06.2021 12:50:35 ]
                                                                                                                    Aloituksessasi kysyit juuri näistä kahdesta henkilöstä   [ 405737 , Trepidaatio , 30.06.2021 16:22:30 ]
                                                                                                                        Aloituksessasi kysyit juuri näistä kahdesta henkilöstä   [ 405739 , Teppolainen , 30.06.2021 16:50:08 ]
                                                                                                                            Aloituksessasi kysyit juuri näistä kahdesta henkilöstä   [ 405740 , Trepidaatio , 30.06.2021 16:55:55 ]
                                                                                                                                Laki sallii pakkotestit, mutta ei kuitenkaan salli?   [ 405742 , Teppolainen , 30.06.2021 23:45:37 ]
                                                                                Edes tartuttajien henkilöllisyyksiä ei rajalla tarkistettu ja myös THL panttaa tietoja - Enqvist varoittaa fasismista   [ 405713 , _Wesa_ , 29.06.2021 14:18:35 ]
                                                                                    Edes tartuttajien henkilöllisyyksiä ei rajalla tarkistettu ja myös THL panttaa tietoja - Enqvist varoittaa fasismista   [ 405715 , Teppolainen , 29.06.2021 14:27:27 ]
                                                                                        Edes tartuttajien henkilöllisyyksiä ei rajalla tarkistettu ja myös THL panttaa tietoja - Enqvist varoittaa fasismista   [ 405729 , _Wesa_ , 29.06.2021 20:36:53 ]
                                                                                Enqvist varoittaa (hyvin) fasismista - mutta ei huomaa itse olevansa NATSI "eurotieteen" "rotumurhapierupeilineurooneineen"...   [ 405735 , Risto Koivula , 30.06.2021 14:49:08 ]
                                                                            Tautia tuodaan taas bussilasteittain ulkomailta   [ 405712 , _Wesa_ , 29.06.2021 14:16:26 ]
                                                                                Tautia tuodaan taas bussilasteittain ulkomailta   [ 405716 , Teppolainen , 29.06.2021 14:33:09 ]
                                                                                    Tautia tuodaan taas bussilasteittain ulkomailta   [ 405728 , _Wesa_ , 29.06.2021 20:28:42 ]
        Totalitaristista hallitusta ei kansanterveys oikeasti kiinnosta   [ 405774 , Teppolainen , 02.07.2021 13:00:56 ]
            Toisen postauksesi linkistä.   [ 405776 , Trepidaatio , 02.07.2021 13:04:45 ]
                Eduskunta ei ole palauttanut lakia voimaan   [ 405777 , Teppolainen , 02.07.2021 13:23:21 ]
                    Toisen postauksesi linkistä:   [ 405778 , Trepidaatio , 02.07.2021 13:31:59 ]
                        Edelleen oleellinen unohtuu   [ 405779 , Teppolainen , 02.07.2021 13:52:43 ]
                            Edelleen oleellinen unohtuu   [ 405781 , Trepidaatio , 02.07.2021 14:12:56 ]
                                Edelleen oleellinen unohtuu   [ 405783 , Teppolainen , 02.07.2021 14:21:04 ]
                                    Virheet korjataan   [ 405784 , Trepidaatio , 02.07.2021 14:31:25 ]
                                        Virheet ja "virheet" korjataan   [ 405787 , Teppolainen , 02.07.2021 14:51:25 ]
            Totalitaristista hallitusta ei kiinnosta maksaa edes terveydenhoidosta vastaavien palkkoja   [ 405943 , Teppolainen , 12.07.2021 20:38:58 ]
                Totalitaristista hallitusta ei kiinnosta maksaa edes terveydenhoidosta vastaavien palkkoja   [ 405947 , _Wesa_ , 12.07.2021 23:27:11 ]
                    Sillä välin Ranska vajoaa totalitarismiin   [ 405949 , Teppolainen , 13.07.2021 01:32:12 ]
                        Sillä välin Ranska vajoaa totalitarismiin   [ 405950 , _Wesa_ , 13.07.2021 02:22:32 ]
                            Sillä välin Ranska vajoaa totalitarismiin   [ 405951 , Teppolainen , 13.07.2021 04:00:41 ]
                                Sillä välin Ranska vajoaa totalitarismiin   [ 405957 , _Wesa_ , 13.07.2021 16:00:05 ]
                                    Sillä välin Ranska vajoaa totalitarismiin   [ 405962 , Teppolainen , 13.07.2021 22:18:40 ]
                                        Sillä välin Ranska vajoaa totalitarismiin   [ 405965 , _Wesa_ , 14.07.2021 11:08:25 ]
                                            Sillä välin Ranska vajoaa totalitarismiin   [ 405970 , Teppolainen , 14.07.2021 15:39:22 ]
                                                Sillä välin Ranska vajoaa totalitarismiin   [ 405975 , _Wesa_ , 15.07.2021 12:24:37 ]
                                                    Sillä välin Ranska vajoaa totalitarismiin   [ 405976 , Teppolainen , 15.07.2021 12:58:14 ]
                                                        Sillä välin Ranska vajoaa totalitarismiin   [ 405977 , _Wesa_ , 15.07.2021 13:11:49 ]
                                                            Sillä välin Ranska vajoaa totalitarismiin   [ 405979 , Teppolainen , 15.07.2021 13:51:42 ]
                                                                Sillä välin Ranska vajoaa totalitarismiin   [ 405983 , _Wesa_ , 15.07.2021 22:05:50 ]
                                                                    Sillä välin Ranska vajoaa totalitarismiin   [ 405985 , Teppolainen , 15.07.2021 22:42:08 ]
                                                                        WHO:n toiminta paljastaa, että kyse ei ole koronasta   [ 405986 , Teppolainen , 16.07.2021 11:04:50 ]
                                                                            Taistelu syrjintää vastaan on alkanut   [ 405989 , Teppolainen , 16.07.2021 16:31:46 ]
                                                                                Ja Yle levittää taas totalitaristisen hallituksen valheita   [ 405990 , Teppolainen , 16.07.2021 17:06:02 ]
                                                                                    Ja Yle levittää taas totalitaristisen hallituksen valheita   [ 406001 , _Wesa_ , 18.07.2021 13:27:49 ]
                                                                                        Nyt jo Ylekin jälleen myöntää valheellisen hysterian   [ 406027 , Teppolainen , 22.07.2021 11:28:55 ]
                                                                                            Nyt jo Ylekin jälleen myöntää valheellisen hysterian   [ 406029 , _Wesa_ , 22.07.2021 12:33:48 ]
                                                                        Sillä välin Ranska vajoaa totalitarismiin   [ 406002 , _Wesa_ , 18.07.2021 13:40:56 ]
                                                                            Demokratian palauttaminen   [ 406015 , Teppolainen , 20.07.2021 10:23:57 ]
                                                                                Demokratian palauttaminen   [ 406016 , _Wesa_ , 20.07.2021 11:17:07 ]
                                                                                    Demokratian palauttaminen   [ 406021 , Teppolainen , 20.07.2021 20:36:47 ]
                                                                                        Demokratian palauttaminen   [ 406022 , _Wesa_ , 21.07.2021 14:49:15 ]
                                                                                            Demokratian palauttaminen   [ 406023 , Teppolainen , 21.07.2021 15:25:50 ]
                                                                                                Demokratian palauttaminen   [ 406024 , _Wesa_ , 21.07.2021 16:11:13 ]
                                                                                                    Demokratian palauttaminen   [ 406025 , Teppolainen , 21.07.2021 16:19:28 ]
                                                                                                        Demokratian palauttaminen   [ 406026 , _Wesa_ , 21.07.2021 16:59:02 ]
                                                                                                            Italia seuraa fasismin jaloa perinnettä - ja Suomi tulossa perässä   [ 406974 , Teppolainen , 17.09.2021 15:06:39 ]
                                                                                                                Jutussa kerrotaan oikeasti...   [ 406976 , Trepidaatio , 17.09.2021 15:20:56 ]
                                                                                                                    Jutussa kerrotaan oikeasti...   [ 406977 , Teppolainen , 17.09.2021 15:49:32 ]
                                                                                                                        Jutussa kerrotaan oikeasti...   [ 406981 , _Wesa_ , 18.09.2021 20:26:45 ]
                                                                                                                            Jutussa kerrotaan oikeasti...   [ 406982 , Teppolainen , 18.09.2021 23:08:46 ]
                                                                                                                                Rokotuspassi on rokotuspassi on syrjintää - naishuijarit taas vedättämässä suomalaisia   [ 406989 , Teppolainen , 20.09.2021 20:25:40 ]
                                                                                                                                    Yle valehtelee jälleen   [ 406995 , Teppolainen , 21.09.2021 15:03:28 ]
                                                                                                                                        Marinin hörhöoppien mukaan pizzansyönnin rajoittamisella lisätään rokotusten määrää   [ 406996 , Teppolainen , 21.09.2021 17:38:46 ]
                                                                                                                                            Nyt myös Suomessa yritetään kytkeä pizzansyöminen rokotuksiin   [ 407121 , Teppolainen , 04.10.2021 15:14:58 ]
                                                                                                                                                Saksaan itsesaastutuskielto sakon uhalla   [ 407125 , Teppolainen , 05.10.2021 15:24:04 ]
                                                                                                                                        Kritiikki natsipääministeri Marinin rokotepassia vastaan kovenee   [ 407117 , Teppolainen , 04.10.2021 02:28:02 ]
                                                                                                        Hallituksen riveistä sataa tunnustuksia Suomen vaalivilpistä   [ 406990 , Teppolainen , 20.09.2021 20:44:36 ]